Revvity, AL182M, AlphaLISA Anti-M13 Acceptor Beads, 5 mg

Overview
AlphaLISA Acceptor beads conjugated to anti-M13 antibody. Anti-M13 beads bind to the G3P Protein of M13 phage. This bead can be used in conjunction with AlphaScreen Donor beads to create no-wash assays. The binding is rapid and stable, making it an ideal choice for use in a variety of assays including peptides, VHH or scFv selection and titration in phage display technology.
Formats
In a typical Alpha PPI or binding assay:

Our 250 µg conditioning allows you to run 500 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.
Our 5 mg conditioning allows you to run 10,000 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.
Our 25 mg conditioning allows you to run 50,000 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.

Features:

No-wash steps, no separation steps
Ease-of-use: few addition steps, fast assay development
Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
How it works
Principle of the Alpha Anti-M13 acceptor beads detection
AlphaLISA is a bead-based assay technology used to study biomolecular interactions in a microplate format. AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Protein-protein interaction brings the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, enabling the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the binding of the 2 partners. In the example shown here, Anti M13-Acceptor bead binds to the M13 phage (partner A), while Tagged partner B* binds to an Anti-Tag Ab labeled with a donor bead.
*partner B can also be biotinylated, Fc fused or unlabeled. In these cases, use the corresponding Alpha reagent (i.e Streptavidin, anti-species, protA, …) labeled with the donor bead for the detection.

Assay protocol of the Alpha Anti-M13 acceptor bead detection
The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a M13 phage expressing a target of interest (Partner A) and a tagged partner B* .Dispense the 2 partners (10 µL), add Anti-M13 acceptor beads (5 µL) and Anti-Tag Antibody labeled with a donor bead (5 µL), then incubate and read.
*partner B can also be biotinylated, Fc fused or untagged. In these cases, use the corresponding ALpha reagent (i.e Streptavidin, anti species, protA,...) labeled with donor beads for the detection.
Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Product Group-Beads
Protocol Time-Overnight at RT
Shipping Conditions-Shipped in Blue Ice
Target-Anti-M13
Technology-Alpha
Unit Size-5 mg