Revvity, AL223C, AlphaLISA Human IL-6 Detection Kit, 500 Assay Points

Overview
Formats:

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half time of an ELISA assay

Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130/ IL-6R receptor complex, leading to activation of JAK/STAT, MAPK and PI3K cascades.
AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-1.3 - 30,000 pg/mL
Limit of Detection-1.3 pg/mL
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-IL6
Target Class-Cytokines
Target Species-Human
Technology-Alpha
Therapeutic Area-Inflammation
Unit Size-500 assay points