Revvity, AL3033HV, AlphaLISA Human BACE-1 Detection Kit, 100 Assay Points

Overview
Formats:

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Beta-secretase 1 (BACE-1) is an aspartic protease encoded by the BACE-1 gene that is expressed ubiquitously throughout the body. BACE-1 has been identified as a necessary enzyme that functions with gamma-secretase to process of the amyloid precursor protein into the amyloid beta peptide, where the BACE-1 enzyme is responsible for the generation of the variable sizes of the peptide, particularly amyloid beta 1-40 and 1-42. As such, BACE1 is thought to be a valuable biomarker for Alzheimer’s disease, especially in the CSF. This AlphaLISA kit allows for the detection and quantification of BACE-1 in human serum, CSF, cell culture media and cell lysates.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-351 - 1,000,000 pg/mL
Limit of Detection-351 pg/mL
Limit of Quantification-1,033 pg/mL
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Blue Ice
Target-BACE-1
Target Class-Biomarkers
Target Species-Human
Technology-Alpha
Therapeutic Area-Oncology
Unit Size-100 assay points