Revvity, AL3108HV, AlphaLISA Human 4-1BBL Detection Kit, 100 Assay Points

Overview
Formats:

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
4-1BBL, also known as CD137L or TNFSF9, is a type II transmembrane receptor protein expressed on activated B cells, monocytes, neutrophils, and neurons. It binds to 4-1BB on activated CD4+ and CD8+ T cells. Ligation of 4-1BBL results in survival of T cells during infection. A soluble form of 4-1BBL has been shown to be cleaved from the surface of activated B cells. There are many drug targets centered on interrupting the binding of 4-1BB to 4-1BBL. These are focused on stimulating immune response to cancers.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-12.6 - 100,000 pg/mL
Limit of Detection-12.6 pg/mL
Limit of Quantification-41.3 pg/mL
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Blue Ice
Target-4-1BBL
Target Class-Biomarkers
Target Species-Human
Technology-Alpha
Therapeutic Area-Oncology
Unit Size-100 Assay Points