Revvity, AL3166C, AlphaLISA Human cIAP / BIR2 Binding Kit, 500 Assay Points

Overview
cIAP1, also called BIRC2, is a member of the Inhibitor of Apoptosis Proteins (IAP) family. IAP proteins are involved in multiple biological processes, such as innate immunity, and play an important role in apoptosis inhibition. cIAP1 overexpression has been associated with cancer resistance, making it an attractive target in cancer therapy where several therapeutic strategies have been investigated, such as SMAC mimetics.
Formats:

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-clAP1 BIR2
Target Class-Binding Assay
Technology-Alpha
Therapeutic Area-Autoimmunity Neuroscience Oncology
Unit Size-500 assay points