Revvity, AL3177C, AlphaLISA AAV2 Detection Kit, 500 Assay Points

Overview
Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 2 was one of the first AAV serotypes studied and used as vectors for specific and natural tropism towards targeted cell types such as neurons and hepatocytes or tissues such as skeletal muscles and the retina. The AAV2 kit is designed to detect and quantify AAV2 particles.
Formats

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 50 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).

Features

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is captured by AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application-Bioprocessing
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-4.5E+06 - 1.0E+11 VP/mL
Limit of Detection-4.5E+06 VP/mL
Limit of Quantification-1.5E+07 VP/mL
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Blue Ice
Target-AAV2
Target Class-Viral Particles
Technology-Alpha
Unit Size-500 assay points