Revvity, AL324F, AlphaLISA Testosterone Detection Kit, 5,000 Assay Points

Overview
Formats:

Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half the time of an ELISA assay

In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. In the presence of high analyte, the beads are separated resulting in lower emission.
Testosterone is the predominant androgenic steroid. This steroid hormone has many functions both in reproductive and non-reproductive tissue. Testosterone plays a key role in the development of testis and prostate. Testosterone levels are also linked with muscle, bone, and body hair growth. Testosterone is observed in most verterbrates, with fish being the largest exception. Testosterone has been used as a treatment for hypogonadism, infertility, osteoporosis, anemia, and low appetite.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-Broad Range: 0.7 - 875 nM/High Sensitivity: 0.035 – 8.5 nM
Limit of Detection-Broad Range: 0.7 nM/High Sensitivity: 0.035 nM
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-Testosterone
Target Class-Biomarkers
Technology-Alpha
Therapeutic Area-Metabolic
Unit Size-5,000 assay points