Revvity, AL582F, AlphaLISA Mouse MCP2 Detection Kit, 5,000 Assay Points

Overview
Formats:

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

No-wash steps, no separation steps
ELISA alternative technology
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours
Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
MCP2 is Monocyte Chemoattractant Protein 2, also known as HC14, CCL8, SCYA10 and SCYA8. It belongs to the monocyte chemoattractant protein (MCP) family. Studies show that unlike all other MCP chemokines, mouse MCP2 is an agonist for the chemokine receptor CCR8 but not for CCR2. Mouse MCP2 induces migration and calcium flux in TH2-R2A cells. Mouse MCP2 is constitutively expressed in the skin and is abundant in skin lymph nodes.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-7.8 - 300,000 pg/mL
Limit of Detection-7.8 pg/mL
Limit of Quantification-25.9 pg/mL
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-MCP2, CCL8
Target Class-Biomarkers
Target Species-Mouse
Technology-Alpha
Therapeutic Area-Inflammation
Unit Size-5,000 assay points