Revvity, AL594HV, AlphaLISA Mouse CXCL1 Detection Kit, 100 assay points

Overview
Mouse CXCL1 is a small peptide chemokine of 8 kDa that belongs to the CXC subfamily. It is also called growth-regulated oncogene-α (GRO-α) and keratinocyte-derived chemokine (KC). It is expressed in neutrophils, macrophages, and epithelial cells. CXCL1 produces its effect via its receptor CXCR2, mainly expressed on neutrophils. Although CXCL1 plays an important role in inflammatory responses by recruiting and activating neutrophils, aberrant expression of CXCL1 has been reported as being associated with the growth and progression of certain tumors such as breast, lung, and gastric cancers.
Formats

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

No-wash steps, no separation steps
ELISA alternative technology
Reliable detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly reliable, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules, triggering a cascade of energy transfer in the Acceptor beads. This results in a sharp peak of light emission at 615 nm.
How it works
Principle of the AlphaLISA Mouse CXCL1 assay
The AlphaLISA Mouse CXCL1 assay is based on an AlphaLISA sandwich immunoassay involving a biotinylated anti-target antibody bound to Streptavidin-coated AlphaLISA Donor beads and an anti-target antibody conjugated to AlphaLISA Acceptor beads. In the presence of the target, both antibodies bind to CXCL1 and the beads come into proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules, triggering a cascade of energy transfer within the Acceptor beads. This results in emission with λmax at 615 nm. The intensity of the signal is directly proportional to the concentration of CXCL1 present in the sample.

 

Protocol of the AlphaLISA Mouse CXCL1 assay
The AlphaLISA CXCL1 assay can be run in a 96- or 384-well detection plate (50 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of mCXCL1 by AlphaLISA reagents. No washing steps are necessary. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
Application-Protein Quantification
Brand-AlphaLISA
Detection Modality-Alpha
Dynamic Range-2 - 15,000 pg/mL
Limit of Detection-0.62 pg/mL
Limit of Quantification-2.18 pg/mL
Lysis Buffer Compatibility-AlphaLISA Assay Buffer 1
Product Group-Kit
Protocol Time-2-step
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target-CXCL1
Target Class-Cytokines
Target Species-Mouse Rat
Technology-Alpha
Therapeutic Area-Immuno-oncology Inflammation Oncology
Unit Size-100 assay points