Revvity, ALSU-PCD3-A-L, AlphaLISA Human Phospho-CD3-ζ (Tyr83) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr83) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were seeded at 500 K/mL and grown overnight in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 3.5 X 106 cells/mL and treated with 44 mM H2O2 for 10 minutes. Cells were lysed with SureFire Ultra Lysis Buffer.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-CD3 ζ
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Therapeutic Area-Autoimmunity Immuno-oncology Oncology
Unit Size-1 vial