Revvity, ALSU-PIRAK-A-L, AlphaLISA Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-IRAK4 (Thr345/Ser346) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Karpas299 cells were seeded at 200K/mL and grown for two days in 100 mL of 10% FBS containing medium. Cells were harvested and washed in HBSS + 0.1% BSA, adjusted to 4 X 106 cells/mL and then treated with 10 ng/mL IL-1β for 10 minutes. Cells were spun down at 1000 g for 5 mins and lysed in 10 mL of SureFire Ultra Lysis Buffer.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-IRAK4
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Therapeutic Area-Autoimmunity Immuno-oncology Inflammation
Unit Size-1 vial