Revvity, ALSU-PP13K-A-L, AlphaLISA Mouse Phospho-PI3K p85 (Tyr458) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PI3 Kinase p85 (Tyr458) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate NIH/3T3 cells were cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-PI3 Kinase p85
Target Class-Phosphoproteins
Target Species-Mouse
Technology-Alpha
Unit Size-1 vial