Revvity, ALSU-PPLCG2-B-L, AlphaLISA Human Phospho-PLC-γ2 (Tyr759) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr759) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106 cells/mL with 5X SureFire Ultra Lysis buffer.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-PLC G2
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Unit Size-1 vial