Revvity, ALSU-PRAB29-A-L, AlphaLISA Human Phospho-RAB29 (Ser72) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RAB29 (Ser72) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K/mL in fresh medium for 24 hours. Cells were treated with 100 ng/mL Calyculin for 2 hours. Cells were spun down at 300 RCF for 5 minutes, washed with HBSS and lysed with Lysis Buffer at 10x106 cells/mL.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-RAB29
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Therapeutic Area-Neuroscience
Unit Size-1 vial