Revvity, ALSU-TIRF7-A-L, AlphaLISA Human Total IRF7 Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total IRF7 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K cells/mL in medium containing 100 nM PMA and incubated for 24 hours. THP-1 derived macrophages were treated with 100 nM Calyculin A for 30 minutes, washed with HBSS and lysed in Lysis Buffer at a final concentration of 8 x 106 cells/mL.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-IRF7
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Therapeutic Area-Inflammation Oncology
Unit Size-1 vial