Revvity, ALSU-TRIP3-B-L, AlphaLISA Mouse Total RIP3 Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Mouse Total RIP3 Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 2 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Total
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-RIP3
Target Class-Phosphoproteins
Target Species-Mouse
Technology-Alpha
Therapeutic Area-Autoimmunity Central Nervous System Neuroinflammation
Unit Size-1 vial