Revvity, ALSU-TVGFR-A-L, AlphaLISA Human Total VEGF Receptor 2 (VEGFR2) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra total VEGFR-2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HUVEC cells were grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes. Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Ultra
Detection Modality-Alpha
Lysis Buffer Compatibility-Lysis Buffer
Molecular Modification-Total
Product Group-Control Lysate
Shipping Conditions-Shipped in Blue Ice
Target-VEGF2 Receptor
Target Class-Phosphoproteins
Target Species-Human
Technology-Alpha
Unit Size-1 vial