Revvity, AP178TB-R, AlphaPlex 545 (Terbium) Anti-6His Acceptor Beads, 25 mg

Overview
AlphaPlex®-545 Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

Protein-protein interaction assays
Protein detection assays
Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay:

250 μg of Acceptor beads is sufficient to run 250-500 wells using a 50 μL reaction volume
5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 μL reaction volume
25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 μL reaction volume Application-Protein-Protein Interaction
Automation Compatible-Yes
Bead Type or Material-AlphaPlex Terbium (545) Acceptor
Brand-AlphaPlex
Conjugates-Anti-6X His
Detection Modality-Alpha
Product Group-Beads
Shipping Conditions-Shipped in Blue Ice
Target Class-Binding Assay
Technology-Alpha
Unit Size-25 mg