Revvity, AP219TB-HV, AlphaPlex 545 (Terbium) Human IL-17A Detection Kit, 100 Assay Points

Overview
The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit contains reagents to run 100 assays, using a 100 µL reaction volume (10 µL of sample). Protocol can be found on the Certificate of Analysis.
AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaPlex
Detection Modality-Alpha
Dynamic Range-55 - 100,000 pg/mL
Limit of Detection-13 pg/mL
Limit of Quantification-55 pg/mL
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Blue Ice
Target-IL17A
Target Class-Cytokines
Target Species-Human
Technology-Alpha
Therapeutic Area-Inflammation
Unit Size-100 assay points