Revvity, AP221TB-F, AlphaPlex 545 (Terbium) Human IL-2 Detection Kit, 5,000 Assay Points

Overview
The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Formats:

Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
Application-Protein Quantification
Automation Compatible-Yes
Brand-AlphaPlex
Detection Modality-Alpha
Dynamic Range-1 - 300,000 pg/mL
Limit of Detection-4.3 pg/mL
Limit of Quantification-19 pg/mL
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-IL2
Target Class-Cytokines
Target Species-Human
Technology-Alpha
Therapeutic Area-Inflammation
Unit Size-5,000 assay points