Revvity, AS119R, AlphaScreen Anti-GST Donor Beads, 25 mg

Overview
Alpha Donor beads conjugated to anti-GST antibody. These beads can be used to capture GST-tagged proteins and peptides, and can be used in conjunction with AlphaLISA, AlphaScreen® or AlphaPlex™ Acceptor beads to create no-wash assays for:

protein-protein interaction assays
protein detection assays
enzymatic assays

Formats
In a typical Alpha PPI or binding assay:

Our 1 mg conditioning allows you to run 2,000 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.
Our 5 mg conditioning allows you to run 10,000 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.
Our 25 mg conditioning allows you to run 50,000 wells in 384-well format, using 20 µg/mL final concentration in a 25 µL assay volume.

Bead concentration can be adjusted for optimal performance.
Features:

No-wash steps, no separation steps
Ease-of-use: few addition steps, fast assay development
Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
How it works
Principle of the Alpha Anti-GST Donor Beads detection
AlphaLISA is a bead-based assay technology used to study biomolecular interactions in a microplate format. AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Protein-protein interaction brings the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, enabling the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the binding of the two partners. In the example shown here, Alpha Anti-GST Donor bead binds to the GST tag, while tagged partner B* binds to an Anti-Tag antibody conjugated to an AlphaLISA, AlphaScreen® or AlphaPlex™ Acceptor bead.
*Partner B can also be biotinylated, 6xHis-tagged, or unlabeled. In these cases, use the corresponding Alpha reagent (i.e Streptavidin, anti-6xHis, protein A, …) conjugated to an Acceptor bead for the detection.

Assay protocol of the Alpha Anti-GST Donor Beads detection

The example describes the protocol using a 20 µL final assay volume for the detection of an interaction between a GST-tagged Partner A and a tagged Partner B* in a presence of a sample (e.g. inhibitor). Dispense Sample (5 µL), add the two partners (10 µL), and a mix of Alpha Anti-GST donor beads and Anti-Tag antibody conjugated to AlphaLISA, AlphaScreen or AlphaPlex  Acceptor beads (5 µL), then incubate and read.
*Partner B can also be biotinylated, 6xHis-tagged, or unlabeled. In these cases, use the corresponding Alpha reagent (i.e Streptavidin, anti-6xHis, protein A, …) conjugated to an Acceptor bead for the detection.
Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-AlphaLISA
Detection Modality-Alpha
Product Group-Beads
Protocol Time-Overnight at RT
Shipping Conditions-Shipped in Blue Ice
Target-Anti-GST
Technology-Alpha
Unit Size-25 mg