Revvity, ASBF-PSYK-A-L, AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) Detection Kit, Control Lysate

Overview
This control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human and Mouse Phospho-SYK (Tyr525/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS + 0.1% BSA and adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.
Application-Cell Signaling
Automation Compatible-Yes
Brand-AlphaLISA SureFire Biotin-Free
Detection Modality-Alpha
Product Group-Control Lysate
Protocol Time-2h at RT
Shipping Conditions-Shipped in Blue Ice
Target-SYK
Target Class-Phosphoproteins
Target Species-Human Mouse
Technology-Alpha
Therapeutic Area-Autoimmunity Oncology
Unit Size-1 vial