Revvity, CP14001, PhenoVue Fluor 400LS - WGA

Overview
WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.
Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.
PhenoVue Fluor 400LS - WGA can be used to visualize cellular membranes in immunofluorescence as well as high-content analysis and screening applications. Its unique photophysical property enables extended multiplexing capability by adding a fifth color while ensuring no spectral overlap, high brightness as well as photostability.
Recommended working concentration: 25 µg/mL
Equivalent number of microplates:

2 - 8 x 96-well microplates
2 - 8 x 384-well microplates
4 - 13 x 1536-well microplates Color-Orange
Form-Lyophilized
Maximum Emission Wavelength (Em-585 nm
Maximum Excitation Wavelength (Ex-395 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Detection Modality-Fluorescence
Filter-Ex: 375-440 nm, Em: 550-650 nm
Fluorophore-PhenoVue ™ Fluor 400LS
Organelle and Cell Compartment-Golgi Apparatus
Quantity-2 x 1 mg
Sample Type-Live and fixed samples
Shipping Conditions-Shipped Ambient
Storage Conditions-2-8 °C, protected from light
Type-Individual reagent