Revvity, CP163, PhenoVue DRAQ5 Total Cell Nuclear Stain, 1 mL

Overview
PhenoVue DRAQ5 Total Cell Nuclear Stain is a lipophilic and membrane permeable far-red fluorescent DNA stain for live or fixed cells. Unlike the routinely used Hoechst 33342, PhenoVue DRAQ5 does not require UV excitation and has little spectral overlap with visible range chromophores. This property makes it readily used for multiplexing applications with other fluorophores even with rhodamine-derived probes. It is spectrally compatible with simple viability dyes such as propidium iodide, and more sophisticated functional probes such as PhenoVue ROS-490 Reactive Oxygen species indicator, PhenoVue 551 Mitochondrial Stain, or PhenoVue Fluor 488 and 555 conjugates, as well as CFP, GFP and YFP tagged proteins. This total cell nuclear stain allows robust cell event counting, while offering nuclear morphology, insights on DNA condensation, fragmentation, and cytoplasmic morphology from a weak differential staining of this compartment. PhenoVue DRAQ5 is preferentially excited by red wavelengths, with a maximum excitation wavelength at 646 nm. Its maximum emission peaks at 697 nm when bound to double stranded DNA. This stain is provided in an aqueous, ready-to-use solution which is usually added as the final step in a staining protocol. It is documented in HCS applications on imaging platforms.
Concentration 5 mM
Recommended working concentration: 5 µM
Equivalent number of microplates:

32-80 x 96-well microplates
32-80 x 384-well microplates
48-96 x 1536-well microplates Color-Far Red
Form-Solution
Maximum Emission Wavelength (Em-697 nm
Maximum Excitation Wavelength (Ex-646 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Detection Modality-Fluorescence
Filter-Cy5
Organelle and Cell Compartment-Nucleus
Quantity-1 x 1 mL (5 µmole)
Sample Type-Live and fixed samples
Shipping Conditions-Shipped Ambient
Storage Conditions-2-8 °C, protected from light (Do not freeze)
Type-Individual reagent