Revvity, CP181, PhenoVue HypoxiTRAK, Hypoxia Indicator

Overview
Oxygen molecules support the intracellular energy production and are used by several biochemical processes. Hypoxia, or low oxygen levels, is therefore a significant stress that often impairs aerobic species' ability to survive and is a key component of the pathological conditions such as inflammation, cardiovascular diseases, and cancer. As such, understanding molecular mechanisms underlying hypoxia inducing conditions is crucial. For example, low oxygen level results in the upregulation of Hypoxia inducible factors (HIFs) which in turn regulate the expression of genes involved in cell survival, angiogenesis, glycolysis, and invasion/metastasis.
PhenoVue HypoxiTRAK is a far-red fluorescent molecule that readily enters live cells. Its spectral characteristics are shared with DRAQ5™.
HypoxiTRAK is converted to a metabolite, under hypoxic conditions, which persists in cells for several days allowing a simple determination of the extent of hypoxia, without antibody staining. It is non-toxic and suited to continuous exposure that allows the generation of hypoxia dependent changes in cell biology. The HypoxiTRAK-metabolite is designed to halt cell cycle progression, preserving the absolute number of hypoxia-experiencing cells. Cells sensitive to cycle arrest induced by the HypoxiTRAK-metabolite may undergo cell death. The non-viable cell fraction is a simple surrogate readout of the hypoxia experience of cells.
Concentration: 10 µM 
Typical working concentration: 50 nM

Equivalent number of microplates:

3-10 x 96-well microplates
3-10 x 384-well microplates
5-16 x 1536-well microplates Color-Far Red
Form-Solution
Maximum Emission Wavelength (Em-697 nm
Maximum Excitation Wavelength (Ex-647 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Cell Function Indicator-Hypoxia indicator
Detection Modality-Fluorescence
Filter-Cy5
Quantity-1 x 500 µL (5 nmoles)
Sample Type-Live samples only
Shipping Conditions-Shipped Ambient
Storage Conditions-2-8 °C, protected from light. (Do not freeze)
Type-Individual reagent