Revvity, CP22R2, PhenoVue Fluor 647 Live Cell Actin Stain - 3x5nmol

Overview
PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments (F-actin) in live cells. Sensitive, rapid and photostable, PhenoVue Fluor 647 live cell actin stain exhibits far-red emission and can be multiplexed with blue, green and orange colors such as PhenoVue nuclear, lysosomal, mitochondrial or tubulin stains.
Like other actin stains derived from jasplakinolide, cytotoxicity can be observed with long exposure time (>24h) which can be significantly limited at concentrations comprised between 30 and 300nM while maintaining high brightness and image quality.

Depending on the cellular model, intracellular retention of PhenoVue Fluor 647 live cell actin stain can be further improved in the presence of efflux pump inhibitor such as PhenoVue Probenecid, Ready to Use Solution.
Typical working concentration: 100 nM
Equivalent number of microplates:

1 to 5 x 96-well microplates

1 to 5 x 384-well microplates

2 to 8 x 1536-well microplates Color-Red
Form-Solution in DMSO
Maximum Emission Wavelength (Em-670 nm
Maximum Excitation Wavelength (Ex-650 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Detection Modality-Fluorescence
Filter-Cy5
Fluorophore-PhenoVue ™ Fluor 647
Organelle and Cell Compartment-Actin
Quantity-3 x 5 nmol
Sample Type-Live and fixed samples
Shipping Conditions-Shipped in Dry Ice
Storage Conditions--16 °C or below, protected from light
Type-Individual reagent