Revvity, CP24051, PhenoVue Fluor 405 – Phalloidin

Overview
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 405 – Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. ​​Its photophysical property enables extended multiplexing capability by adding a fifth color while avoiding spectral overlap and providing high brightness as well as photostability.
Quantity per pack: 300 units (10 nmoles; 11 µg)
Suggested working concentration: 165 nM (0.17 µg/mL)
Equivalent number of microplates:

2 - 6 x 96-well microplates
2 - 6 x 384-well microplates
3 - 10 x 1536-well microplates Color-Blue
Form-Desiccated
Maximum Emission Wavelength (Em-452 nm
Maximum Excitation Wavelength (Ex-410 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Detection Modality-Fluorescence
Filter-Ex: 375-430 nm, Em: 440-500 nm
Fluorophore-PhenoVue ™ Fluor 405
Organelle and Cell Compartment-Actin
Quantity-1 x 300 units
Sample Type-Fixed samples only
Shipping Conditions-Shipped in Dry Ice
Storage Conditions--16 °C or below, protected from light
Type-Individual reagent