Product Description
Overview
PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 455 nm. It is commonly used to stain cells' nuclear compartment.
PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Typical working concentration: 2 µg/mL (3.24 µM)
Equivalent number of microplates:
170 - 520 x 96-well microplates
140 - 520 x 384-well microplates
270 - 800 x 1536-well microplates
See Product Information sheet for more information on fixed cell staining. Color-Blue
Concentration-10 mg/mL (16.23 mM)
Form-Solution in dd H2O
Maximum Emission Wavelength (Em-455 nm
Maximum Excitation Wavelength (Ex-357 nm
Application-High Content Imaging Microscopy
Brand-PhenoVue™
Detection Modality-Fluorescence
Filter-DAPI
Organelle and Cell Compartment-Nucleus
Quantity-1 x 10 mg
Sample Type-Live and fixed samples
Shipping Conditions-Shipped Ambient
Storage Conditions-2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
Type-Individual reagent
Order Guidelines
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2. Please DO NOT make payment before confirmation.
3. Minimum order value of $1,000 USD required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924