Revvity, ES-600-M400UA, Membrane Target Systems: Gonadotropin Releasing Hormone (human) membrane preparation in CHO-K1 cells, 400 Units

Overview
Membrane Target Systems are quality assured frozen membranes from cells that express human Gonadotropin Releasing Hormone (GnRH) recombinant or endogenous receptors.

We submit every batch of receptors to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi coupled receptors.

Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either bead based proximity methods or classical filtration methods).

Products are packaged as frozen crude membrane preparations in storage buffer. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Technical Data Sheet. Concentration-10 µg/µL protein
Volume-400.0 µL
µg protein/unit-10 µg protein / unit
Application-Drug Discovery & Development
Brand-Membrane Target Systems
Buffer/Solvent-50 mM Tris-HCL (pH 7.4), 0.5mM EDTA, 10mM MgCl2, 10% sucrose
G-Alpha Natural Receptor-G i /G o
Host Cell Line-CHO-K1
Host Species-Human
Product Group-Membrane Preparation
Receptor-Hormone
Shipping Conditions-Shipped in Dry Ice
Target Class-GPCR
Unit Size-400 units
Validation-Binding