Revvity, NEL544001EA, Biotin-11-ATP

Overview
This biotin labeled analog of adenosine triphosphate (ATP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Biotin labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Biotin-labeled products may be detected with streptavidin conjugates.
The number "11" in the compound name refers to the length of the linker arm between ATP and the label.

ATP labeling: Biotin and a fluorescent dye are attached to an ATP molecule through a flexible linker.
Enzymatic incorporation: During in vitro transcription, RNA polymerases utilize the labeled ATP to incorporate the modified nucleotide into the elongating RNA strand, embedding the biotin and fluorescent markers directly into the probe.
Probe detection: Once transcription is complete, the RNA probes carrying both biotin and fluorescent tags can be identified and tracked using appropriate detection methods.
Biotin-Streptavidin binding: The biotin moiety on the RNA probe binds with high specificity and strength to streptavidin, a protein known for its exceptional affinity for biotin.
Fluorescence detection: Streptavidin conjugated to a fluorescent dye attaches to the biotin-labeled RNA, allowing for visualization under fluorescence microscopy or other detection system. Concentration-1 mM
Volume-25.0 µL
Buffer/Solvent-10 mM Tris-HCl, pH 7.6, 1 mM EDTA
Conjugates-Biotin
Detection Modality-Indirect
Nucleotide-ATP
Shipping Conditions-Shipped Ambient
Unit Size-25 nmol