Revvity, NOVA-4202-05, NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits, Barcodes 97-192

Overview
The NEXTFLEX 16S V1–V3 Amplicon-Seq Kit delivers reliable microbial community profiling from a region preferred in many clinical research, environmental, and legacy workflows. Its robust two-step design, flexible indexing, and analysis-ready outputs streamline library prep and hand off cleanly to Cosmos-Hub or your existing 16S analysis pipeline.

Broad profiling: Amplifies the V1–V3 hypervariable regions for comprehensive bacterial coverage with strong clade-level resolution

Straightforward workflow: Two PCR steps with minimal cleanups, custom sequencing primers are not needed

Beads included: NEXTFLEX NGS Cleanup Beads are supplied for both cleanups

Flexible input: Validated for 1–50 ng DNA to accommodate variable sample quality

Scalable multiplexing: Indexing sets are available for projects ranging from small pilot studies to large-scale surveys, with up to 384 unique PCR II barcodes

Automated options: Compatible with Sciclone NGS and NGSx workstations

Built-in analysis: product variants with “S” in their SKU number include Cosmos-Hub® access for DADA2-based ASV analysis with SILVA taxonomy Analysis-Not Included
Automation Compatible-Yes
Barcodes-97 - 192
Product Group-16S amplicon-seq
Shipping Conditions-Shipped in Dry Ice
Unit Size-192 rxns