Revvity, NOVA-4203-05S, NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

Overview
Straightforward workflow: two PCR steps with minimal cleanups; no custom sequencing primers required.
Cleanup beads included: NEXTFLEX NGS Cleanup Beads are supplied with the kit, allowing PCR cleanups without purchasing separate beads. This reduces consumables cost and simplifies purchasing.
Accurate taxonomic assignment: overlapping paired-end 2×300 reads across the V4 region on Illumina® MiSeq® and Element® Aviti® sequencers support robust error correction and ASV recovery.
Flexible Input: validated conditions for 1–50 ng genomic DNA help maintain consistency across samples.
Multiplexing Options: from 12 to 384 PCRII Barcodes are available to support projects of all sizes.
Analysis: product variants with “S” in their SKU include Cosmos-Hub 16S analysis (DADA2 with SILVA) to generate quality-controlled ASV tables and interactive reports.
Automation: protocols are available for Sciclone™ NGS, NGSx, and Sciclone G3 NGSx iQ™ Workstations to automate 16S sequencing.

Use this kit when you want a dependable 16S V4 library prep that fits standard 2×300 sequencing on Illumina® MiSeq® or Element® Aviti® sequencers and hands off cleanly to downstream taxonomic analysis, including the Cosmos-Hub. Analysis-Included
Automation Compatible-Yes
Barcodes-97 - 192
Product Group-16S amplicon-seq
Shipping Conditions-Shipped in Dry Ice
Unit Size-192 rxns