Revvity, NOVA-4204-04S, NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit

Overview
Straightforward workflow: two PCR steps with minimal cleanups; no custom sequencing primers required.

Beads included: NEXTFLEX NGS Cleanup Beads come in the kit, so PCR cleanups are covered without a separate purchase, which can reduce consumables spend and simplify ordering.

Confident taxonomy calls: overlapping paired-end 2×300 reads across the V3-V4 amplicon on Illumina MiSeq and Element Aviti enable strong error correction and ASV recovery.

Flexible Input: validated conditions for 1 – 50 ng genomic DNA help maintain consistency across samples.

Scalable indexing: choose from 48 to 96 PCR II barcodes (384 available upon request) to match project size.

Built-in analysis option: SKUs with an “S” include Cosmos-Hub 16S analysis using DADA2 with SILVA, producing quality-controlled ASV tables and interactive reports.

Automation ready: methods available for Sciclone NGS and NGSx workstations, including G3 NGSx iQ.

Use this kit when you need reliable 16S V3-V4 library prep for standard 2×300 paired-end runs on Illumina MiSeq or Element Aviti, with a clean handoff to downstream analysis in Cosmos-Hub. Analysis-Included
Automation Compatible-Yes
Barcodes-1 - 96
Product Group-16S amplicon-seq
Shipping Conditions-Shipped in Dry Ice
Unit Size-192 rxns