Revvity, NOVA-5149-21, NEXTFLEX Rapid XP V2 DNA-Seq Kit

Overview
Single-tube enzymatic fragmentation, end-repair & A-tailing - DNA to sequencing-ready libraries in as little as 2.5 hours
PCR-free option for ≥ 250 ng input plus a broad 100 pg - 1 µg range for low- and high-input samples with PCR amplification
Built-in NEXTFLEX normalization beads - skip post-library quantitation and manual pooling
Ultra-low adapter-dimer formation & uniform GC coverage for confident variant detection
Up to 1,536 UDIs available for large, multiplexed studies
96 UDI-UMI barcode set enables molecular error-correction for rare variant calling
Automation-ready workflow validated on Revvity Automation NGS workstations
Quality assured through rigorous testing of every lot

The NEXTFLEX Rapid XP V2 DNA-Seq Kit unites speed, data quality, and scalability: enzymatic fragmentation streamlines the workflow, proprietary normalization beads remove an entire QC step, and an optional PCR-free path preserves library complexity. Whether processing a handful of samples or a 96-well plate on a liquid-handler, you get consistent, unbiased libraries ready for Illumina® and Element AVITI™ sequencing. Automation Compatible-Yes
Product Group-DNA-seq
Shipping Conditions-Dual Temperature
Unit Size-8 rxns