Revvity, NOVA-801100, NEXTFLEX Cas9-gRNA rRNA Depletion Enzyme (HMR)

Overview
Ribosomal RNA can account for more than 90% of reads in total RNA sequencing libraries, making depletion essential for detecting low-abundance, biologically relevant transcripts. The NEXTFLEX Cas9-gRNA rRNA Depletion Enzyme (HMR) is a cost-effective, workflow-independent, and highly specific enzyme that boosts usable reads without requiring you move to a new library prep kit. It implements a DASH (Depletion of Abundant Sequences by Hybridization) CRISPR depletion approach, using a precomplexed Cas9 and a pool of guides to cleave human, mouse, and rat rRNA sequences at the dsDNA stage. Whether you use your own prep method or a third-party kit, this module gives you clean, reproducible results backed by the performance of DepleteX™ technology.

Powered by Jumpcode™ technology for specific rRNA removal.
Ready-to-use: Provided as a Cas9-gRNA complex with integrated RNase inhibitor and Cas9 Buffer
Fast: Short one hour reaction time with only 5 minutes of hands-on time
Convenient: Easily fit into any library prep workflow
Flexible: Compatible with short and long-read sequencing
Automation-compatible – easily incorporated into manual or automated workflows Automation Compatible-Yes
Product Group-RNA Depletion
Shipping Conditions-Shipped in Dry Ice
Unit Size-50 μL