Revvity, NOVA-802100, NEXTFLEX Cas9-gRNA Globin Depletion Enzyme (Human)

Overview
Globin transcripts can account for a large fraction of reads in whole blood RNA sequencing libraries, which can mask low-abundance, biologically relevant transcripts. The NEXTFLEX Cas9-gRNA Globin Depletion Enzyme (Human) is a cost-effective, workflow-independent, and highly specific enzyme that reduces globin-derived reads, so more sequencing capacity is directed toward informative genes, without requiring you to move to a new library prep kit.
It uses a DASH-style (Depletion of Abundant Sequences by Hybridization) CRISPR-Cas9 depletion approach, with a precomplexed Cas9 and pool of guides that cleave human globin genes at any dsDNA stage. Whether you use your own prep method or a commercial kit, this module gives you clean, reproducible depletion of globin sequences and improved coverage of non-globin transcripts.

Cas9-gRNA-mediated targeted removal of human globin genes
Ready-to-use: provided as a Cas9-gRNA complex with integrated RNase inhibitor and 10X Cas9 Buffer
Fast: short one-hour reaction time with only 5 minutes of hands-on time
Convenient: easily fits into any library prep workflow
Flexible: compatible with short- and long-read sequencing
Automation-compatible: easily incorporated into manual or automated workflows Automation Compatible-Yes
Product Group-RNA Depletion
Shipping Conditions-Shipped in Dry Ice
Unit Size-50 μL