Revvity, PHTA2A, Tag-lite pHaloTag-A2A Plasmid, 10 µg

Overview
Over past few years, SNAP/HALO-Tag technology combined with TR-FRET has paved way development of many non-radioactive, no-wash, binding assays.
method is based on transfecting cells using plasmids encoding a SNAP- or HALO-Tag and subsequently labeling m with Terbium. Revvity offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready protein expression and labeling.
All information on this page pertains to Tag-lite plasmid cloned with A2A Adenosine receptor.
Tag-lite assays offer many features and applications over traditional receptor/ligand binding assays:

Non-radioactive
Homogeneous and filtration-free
Ready-to-use kits and reagents for binding assays
Does not alter the receptor pharmacology
Peer-reviewed, validated technology
Thorough kinetic studies with true Kd and Ki values, association (Kon) and dissociation (Koff) rate constants
Screening and profiling of biologics and large molecules Application-Receptor-Ligand Binding
Brand-Tag-lite
Detection Modality-HTRF
Product Group-Plasmids
Shipping Conditions-Shipped in Dry Ice
Target Class-GPCR
Technology-TR-FRET
Therapeutic Area-Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size-1 each