Revvity, RBHS3M400UA, Membrane Target Systems: Serotonin 5HT3 (human) membrane preparation

Overview
Membrane Target Systems are quality assured frozen membranes from cells that express Serotonin 5-HT3 (human) recombinant or endogenous receptors.
We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi-coupled receptors.
Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtration methods).
Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet.
Some of our receptors may be restricted for sale in specified countries. Please inquire. Concentration-2.5 µg/µL protein
Volume-400.0 µL
µg protein/unit-2.5 µg protein / unit
Application-Drug Discovery & Development
Brand-Membrane Target Systems
Buffer/Solvent-50 mM Tris-HCL (pH 7.4), 0.5mM EDTA, 10mM MgCl2, 10% sucrose
Host Cell Line-HEK293
Host Species-Human
Product Group-Membrane Preparation
Receptor-5-Hydroxytryptamine (Serotonin)
Shipping Conditions-Shipped in Dry Ice
Target Class-Ion channel
Therapeutic Area-Central Nervous System Inflammation
Unit Size-400 units
Validation-Binding