Revvity, TRF0206-M, LANCE Anti-Phospho-IκBα (Ser32/36) Eu-Antibody, 100 µg

Overview
LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

Test conditions: Rapid assay setup to move straight into optimized assay performance
S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
Phospho-motifs: Substrate sequences are provided
Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody

10 μg = 1,562 assay points
100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation. Application-Biochemical Enzymatic Assay
Automation Compatible-Yes
Brand-LANCE Ultra
Conjugates-Anti-phospho-IkappaBa
Detection Modality-LANCE
Fluorophore-Eu-W1024
Molecular Modification-Phosphorylation
Product Group-Antibody
Shipping Information-Blue Ice
Shipping Conditions-Shipped in Blue Ice
Target Class-Kinase
Technology-TR-FRET
Therapeutic Area-Autoimmunity Immuno-oncology Inflammation
Unit Size-100 µg