Revvity, TRF0414-D, LANCE Ultra Anti-Methyl-R3 Histone H4 Eu-Antibody, 10 µg

Overview
The LANCE® Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) antibody was used for the development and optimization of a PRMT1 methyltransferase assay using a biotinylated Histone H4 peptide as substrate. A technical note describing the assay is available in our product literature.
Formats:

10 µg = 1,500 assay points (384-well assay format)
100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification. Application-Biochemical Enzymatic Assay
Automation Compatible-Yes
Brand-LANCE Ultra
Conjugates-Anti-H4R3me
Detection Modality-LANCE
Fluorophore-Eu-W1024
Molecular Modification-Methylation
Product Group-Antibody
Shipping Conditions-Shipped in Dry Ice
Target Class-Epigenetics
Technology-TR-FRET
Unit Size-10 µg