Revvity, TRF1111C, LANCE Ultra Human c-Myc Binding Kit, 500 Assay Points

Overview
The LANCE Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight™ dye]. A “tracer” analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.
The c-myc polypeptide tag is derived from the c-myc gene. The tag can be used during affinity chromatography and to separate wild-type protein from recombinant protein. The c-myc tag can be used in indirect detection assays for which there is no antibody for the target. Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-46.1- 300,000 nM
Limit of Detection-46.1 nM
Limit of Quantification-235.8 nM
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-c-Myc
Target Class-Binding Assay
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology
Unit Size-500 assay points