Revvity, TRF1201M, LANCE Human VEGF Detection Kit, 10,000 Assay Points

Overview
The LANCE® Ultra Human VEGF Detection Kit is designed for detection and quantitation of human VEGF in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Vascular endothelial growth factor (VEGF-A or VEGF) is a homodimeric 34-42 kDa heparin-binding glycoprotein specific for endothelial cells. Currently at least eight variants of VEGF generated by alternative splicing of the same gene have been identified. The various isoforms have different heparin- and neuropilin(-1 or -2)-binding properties as well as solubility characteristics reflecting the different functional properties of the VEGF forms. VEGF is believed to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. Elevated levels of VEGF have been observed in synovial fluids of rheumatoid arthritis patients and sera of cancer patients. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-19.5 - 100,000 pg/mL
Limit of Detection-19.5 pg/mL
Limit of Quantification-99.5 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-VEGF-A
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology
Unit Size-10,000 assay points