Revvity, TRF1208C, LANCE Human TNF-α Detection Kit, 500 Assay Points

Overview
The LANCE® Ultra Human TNFα Detection Kit is designed for detection and quantitation of human TNFα in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Tumor necrosis factor alpha (TNFα) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble homotrimeric complex. TNFα plays a role in lipid metabolism, coagulation, and endothelial function and has been associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury and heart failure, and insulin resistance. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-9.3- 30,000 pg/mL
Host Species-Human
Limit of Detection-9.3 pg/mL
Limit of Quantification-47.4 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-TNFα
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-500 assay points