Revvity, TRF1257M, LANCE Human CCL3 Detection Kit, 10,000 Assay Points

Overview
The LANCE® Ultra Human CCL3/MIP1α Detection Kit is designed for detection and quantitation of human CCL3 (MIP1α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
C-C Motif Chemokine 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1α), contains 70 amino acids in its mature form. It is a factor produced by macrophages that causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. CCL3 is involved in the acute inflammatory state and in the recruitment and activation of polymorphonuclear leukocytes. It binds to CCR1, CCR4, and CCR5. CCL3 is known mainly for its activity as a chemotactic cytokine, but may also have a potential role in host defense as a direct antiviral agent. CCL3 has been reported to show a significant direct antiviral activity against HSV-1. It is one of the major HIVsuppressive factors produced by CD8+ T-cells. CCL3 is also an inhibitor of haematopoietic stem cell proliferation. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-5.3 - 100,000 pg/mL
Host Species-Human
Limit of Detection-5.3 pg/mL
Limit of Quantification-22.5 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-CCL3~MIP1α
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-10,000 assay points