Revvity, TRF1351M, LANCE Human Fibronectin Detection Kit, 10,000 Assay Points

Overview
The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Fibronectin is a large glycoprotein of the extracellular matrix that exists in two forms: insoluble (cellular) and as a soluble plasma protein. The primary functions of fibronectin are its involvement in cell adhesion, growth, migration, and differentiation. Fibronectin is known to contribute to the wound healing process where it will accumulate at the site of injury to help form blood clots and aid in the development of new tissues. Fibronectin levels in plasma have been identified to increase significantly after major traumas. Further, Fibronectin levels have been observed to be significantly altered in disease states of certain types of cancer and also diabetes mellitus. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-70 - 300,000 pg/mL
Limit of Detection-70 pg/mL
Limit of Quantification-380 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-Fibronectin
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology
Unit Size-10,000 assay points