Revvity, TRF1366M, LANCE Human EGF / EGFR Detection Kit, 10,000 Assay Points

Overview
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. Streptavidin is labeled with a donor fluorophore (a LANCE Europium chelate) which binds to biotinylated EGF and the EGFR-Fc is recognized by anti-human IgG labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Epidermal Growth Factor Receptor (EGFR, Her1, ErbB1) is a 134 kDa cell surface receptor, part of a four member subfamily of receptor tyrosine kinases (erbB1, erbB2, erbB3, and erbB4). EGFR binds to a family of proteins called the epidermal growth factors (primarily EGF), which, upon association induces dimerization of the receptor and initiates signal transduction for the promotion of cell survival and growth. Mutations that cause aberrant overexpression of EGF and/or EGFR have been linked with many different cancers such as non-small-cell lung and colon cancers. Drugs are being developed as a means to better treat these types cancer. Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Blue Ice
Target-EGF,EGFR
Target Class-Binding Assay
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology
Unit Size-10,000 assay points