Revvity, TRF1396C, LANCE Human ApoC3 Detection Kit, 500 Assay Points

Overview
The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Apolipoprotein CIII (ApoCIII), a relatively small protein (79 amino acids), is a component of very low density lipoproteins. Apo-CIII delays the catabolism of triglyceride rich particles by inhibiting the activities of lipoprotein lipase, hepatic lipase, and blocks the uptake of triglyceride-rich particles by the cells. Therefore, ApoCIII has been associated with coronary heart disease and diabetes. An increase in ApoCIII levels induces the development of hypertriglyceridemia and associated with non-alcoholic fatty liver disease. Application-Protein Analysis & Detection
Assay Points-500
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-TR-FRET
Format-Microplates
Shipping Conditions-Shipped in Blue Ice
Target-ApoC3
Target Class-Protein
Therapeutic Area-Metabolic
Unit Size-500 Assay Points