Revvity, TRF3016C, LANCE Human ANGPTL3 Detection Kit, 500 Assay Points

Overview
The LANCE® Ultra Human ANGPTL3 Detection Kit is designed for detection and quantitation of human angiopoietin-like 3 protein (ANGPTL3) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Angiopoietin-like 3, also known as ANGPTL3, is a glycoprotein that is structurally related to other members of the angiopoietin family. It is expressed predominantly in the liver, and contains a signal peptide, an N-terminal coiled-coil domain, and a C-terminal fibrinogen-like domain. Angiopoietin-like 3 is a determinant factor of HDL levels and positively correlates with plasma HDL cholesterol. It may also play a role in the regulation of angiogenesis. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-21.0- 300,000 pg/mL
Limit of Detection-21.0 pg/mL
Limit of Quantification-113.1 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-ANGPTL3
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology
Unit Size-500 Assay Points