Revvity, TRF3039M, LANCE Human IFN-λ2 Detection Kit, 10,000 Assay Points

Overview
The LANCE® Ultra Human IFN-λ2 Detection Kit is designed for detection and quantitation of human interferon λ2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Interferon-λ2 (IFN-λ2), also know an IL28A is a cytokine encoded by the IL28 gene and is closely related to IL28B (IFN-λ3) and IL29(IFN-λ1). IFN-λ2 and IFN-λ3 are 96% homologous and their functional differences are still unknown. IFN-λ2 plays a critical role in adaptive immune responses and regulates CD8+ T cell functions during vaccination. Clinically, it is involved in the protection from H1N1 viral infection and it can be used for predicting response to hepatitis C treatment with interferon and ribavirin. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-63.2 - 100,000 pg/mL
Limit of Detection-63.2 pg/mL
Limit of Quantification-616.8 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-IFN-λ2
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-10,000 Assay Points