Revvity, TRF4021C, LANCE Phospho-BTK (Tyr223) Detection Kit, 500 Assay Points

Overview
Please note control lysates are sold separately, catalog number TRF4021S.
Formats:

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
BTK is a cytoplasmic tyrosine kinase present in B-cells. It is associated with the B-cell receptor and receptors of the toll family. Upon activation, BTK will activate the Nf-kB pathway. This will induce maturation of B-cells into antibody producing cells and also increase the secretion of activating cytokines (such as IL-2). The protein is absent or non-functional in diseases such as aglobularia, but is also involved by hyperactivation in inflammation and auto-immune diseases. Also, several leukemia-like cancers have dysregulation of BTK. Application-Cell Signaling
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Molecular Modification-Phosphorylation
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-BTK
Target Class-Phosphoproteins
Technology-TR-FRET
Therapeutic Area-Autoimmunity Immuno-oncology Neuroscience Oncology
Unit Size-500 Assay Points